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KMID : 0356620090240020093
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Journal of Korean Society of Endocrinology
2009 Volume.24 No. 2 p.93 ~ p.99
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Search for Materials that Influence Human Medullary Thyroid Carcinoma Cell Proliferation
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Shin Hyun-Won
Jang Hye-Won
Kim Keun-Sook
Lee Ji-In
Park Ji-Young
Kim Sun-Wook
Min Yong-Ki
Lee Myung-Shik
Lee Moon-Kyu
Kim Kwang-Won
Chung Jae-Hoon
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Abstract
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Background:Surgical excision is the only effective treatment of medullary thyroid carcinoma (MTC) and there is no certain treatment for recurrence or distant metastasis. Materials that influence MTC cell proliferation were recently reported. Presently, we evaluated the influence of dexamethasone, somatostatin, progesterone, estradiol-17-beta, forskolin and gastrin on MTC cell proliferation and calcitonin secretion.
Methods:Genomic DNA was extracted and sequenced from untreated thyroid TT cells and cells treated with 10-5~10-10 M dexamethasone, somatostatin, progesterone, estradiol-17-beta, forskolin or gastrin, and cultured for 1~6 days. Cell proliferation was assessed using a BrdU assay at days 1, 2, 3, and 6. Calcitonin in the culture medium from dexamethasone-treated TT cells was measured at days 1~3.
Results:Replacement of cysteine with tryptophan at codon 634 of exon 11 was evident in treated TT cells. There was no significant difference in cell proliferation at days 1~3 in cells treated with somatostatin, progesterone, estradiol-17-beta, gastrin and forskolin, while proliferation was inhibited in dexamethasone-treated cells in a concentration-dependent manner from 10-5~10-8 M with no inhibition evident at 10-10 M. Calcitonin levels in 10-5~10-8 M dexamethasone-treated cells were decreased.
Conclusion:Dexamethasone is a potentially useful compound to suppress MTC cell proliferation. Further studies are necessary to explore this potential further prior to clinical use.
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KEYWORD
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calcitonin, dexamethasone, estradiol-17-beta, forskolin, gastrin, medullary thyroid carcinoma, progesterone, somatostatin
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